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RESEARCH PAPER
There is no correlation between the functional polymorphism S460C>T of vascular endothelial growth factor (VEGF) gene promoter and uncomplicated recurrent urinary tract infection among young women
| 1 | Department of Internal Medicine, School of Medicine, Syiah Kuala University, Banda Aceh, Indonesia |
| 2 | Medical Research Unit, School of Medicine, Syiah Kuala University, Banda Aceh, Indonesia |
| 3 | Tropical Diseases Centre, School of Medicine, Syiah Kuala University, Banda Aceh, Indonesia |
| 4 | Department of Microbiology School of Medicine, Syiah Kuala University, Banda Aceh, Indonesia |
| 5 | Department of Internal Medicine, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia |
| 6 | Department of Histology and Cell Biology, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia |
| 7 | Department of Pharmacology, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia |
CORRESPONDING AUTHOR
Maimun Syukri
Jl. T. Tanoeh Abe, Darussalam, Banda Aceh 23111, Indonesia. Tel.: +62 811 685 945; fax: +62 651 26090.
Jl. T. Tanoeh Abe, Darussalam, Banda Aceh 23111, Indonesia. Tel.: +62 811 685 945; fax: +62 651 26090.
Submission date: 2014-10-13
Acceptance date: 2015-03-12
Online publication date: 2015-04-19
Publication date: 2020-03-24
Pol. Ann. Med. 2015;22(1):5–10
KEYWORDS
ABSTRACT
Introduction:
The –460C>T polymorphism in vascular endothelial growth factor (VEGF) gene promoter (rs833061) has robust association with various diseases including renal parenchymal scarring following urinary tract infection (UTI). However, the association of this polymorphism with uncomplicated recurrent UTI (RUTI) is still unclear.
Aim:
The objective of this study was to assess the correlation between VEGF –460C>T polymorphism and uncomplicated RUTI among young women.
Material and methods:
Polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) with BstU1 restriction enzyme – and DNA sequencing were used to determine the VEGF –460C>T polymorphism among 34 young adult women with uncomplicated RUTI and 34 healthy controls. Differences in genotype and allele frequencies between case and control groups were analyzed with x2 test or Fisher's exact test.
Results and discussion:
This study found that there was no significant difference in genotype frequencies between cases (CC 23.5%; CT 26.5%; TT 0%) and controls (CC 85.3%; CT 14.7%; AA 0%). Dominant model analysis found that there was no significant difference between uncomplicated RUTI and normal groups (P = 0.368). Similarly, allele analysis also found that there was no association between VEGF –460C>T polymorphism and uncomplicated RUTI (P = 0.398).
Conclusions:
This study found that there was no correlation between VEGF –460C>T polymorphism and uncomplicated RUTI among young women.
The –460C>T polymorphism in vascular endothelial growth factor (VEGF) gene promoter (rs833061) has robust association with various diseases including renal parenchymal scarring following urinary tract infection (UTI). However, the association of this polymorphism with uncomplicated recurrent UTI (RUTI) is still unclear.
Aim:
The objective of this study was to assess the correlation between VEGF –460C>T polymorphism and uncomplicated RUTI among young women.
Material and methods:
Polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) with BstU1 restriction enzyme – and DNA sequencing were used to determine the VEGF –460C>T polymorphism among 34 young adult women with uncomplicated RUTI and 34 healthy controls. Differences in genotype and allele frequencies between case and control groups were analyzed with x2 test or Fisher's exact test.
Results and discussion:
This study found that there was no significant difference in genotype frequencies between cases (CC 23.5%; CT 26.5%; TT 0%) and controls (CC 85.3%; CT 14.7%; AA 0%). Dominant model analysis found that there was no significant difference between uncomplicated RUTI and normal groups (P = 0.368). Similarly, allele analysis also found that there was no association between VEGF –460C>T polymorphism and uncomplicated RUTI (P = 0.398).
Conclusions:
This study found that there was no correlation between VEGF –460C>T polymorphism and uncomplicated RUTI among young women.
CONFLICT OF INTEREST
There is no conflict of interest.
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