Evaluation of dentinal tubules of dentin of the roots of necrotic teeth by means of scanning electron microscope
More details
Hide details
Chair and Department of Oral Surgery, Medical University of Lublin, Poland
Department of Chromatographic Methods, Maria Curie-Sklodowska University, Lublin, Poland
Submission date: 2016-07-08
Acceptance date: 2016-11-16
Online publication date: 2016-12-07
Publication date: 2019-12-15
Corresponding author
Joanna Jakiel   

Chair and Department of Oral Surgery, Medical University of Lublin, Karmelicka 7, 20-081 Lublin, Poland. Tel.: +48 604 113 [1TD$DIF]793; fax: +48 815 287 951.
Pol. Ann. Med. 2017;24(1):36-42
Dentin is porous due to existence of tubules, containing cell knobs and nerve fibers. It forms integral part of the tooth.

The aim of the study was measurement of the width of dentinal tubules of the roots of necrotic teeth with chronic periapical inflammation after endodontic treatment as well as differences related to age and gender.

Material and methods:
The study comprised 53 teeth (30 molars, 23 premolars) extracted at the Oral Surgery Department, Medical University of Lublin.The teeth were divided into two groups: 40 teeth extracted because of periapical lesions, 15 of which had been treated endodontically before, and the control group of 13 healthy teeth extracted for orthodontic reasons. Scanning electron microscope was used to evaluate the construction of dentinal tubules. The diameter of dentinal tubules was measured close to the cementum and near the dentinocemental junction.

Results and discussion:
The study demonstrated larger diameter of dentinal tubules in necrotic teeth roots compared to the width of tubules of vital teeth. In the control group the mean tubule diameter near the cement was 1.38 µm, and nearby the root canal 3.10 µm. In the group of devitalised teeth the average diameter measured in the same position amounted to 3.68 µm and 4.89 µm.

The width of dentinal tubules of necrotic teeth was significantly higher compared to the teeth with vital pulp. Endodontic treatment does not change the width of dentinal tubules. The width of dentinal tubules does not depend on age and gender.

None declared.
Boskey AL. Mineralization of bone and teeth. Element. 2007;3(6):385–391.
Antonova IN, Goncharov VD, Kipchuk AV, Bobrova EA. Evaluation of dental hard tissues by means of atomic force microscopy. Stomatologiia (Mosk). 2014;93(4):11–14.
Moller IJ. Influence of microelements on the morphology of the teeth. J Dent Res. 1967;46(5):933–937.
Morita W, Yano W, Nagaoka T, Abe M, Ohshima H, Nakatsukasa M. Patterns of morphological variation in enamel-dentin junction and outer enamel surface of human molars. J Anat. 2014;224(6):669–680.
Komabayashi T, Nonomura G, Watanabe LG, Marshall Jr GW, Marshall SJ. Dentin tubule numerical density variations below the CEJ. J Dent. 2008;36(11):953–958.
Charoenlarp P, Wanachantararak S, Vongsavan N, Matthews B. Pain and the rate of dentinal fluid flow produced by hydrostatic pressure stimulation of exposed dentine in man. Arch Oral Biol. 2007;52(7):625–631.
Viţalariu A, Căruntu ID, Bolintineanu S. Morphological changes in dental pulp after the teeth preparation procedure. Rom J Morphol Embryol. 2005;46(2):131–136.
Aguiar TR, Tristao GC, Mandarino D, Zarranz L, Ferreira VF, Barboza EP. Histopathologic changes in dental pulp of teeth with chronic periodontitis. Compend Contin Educ Dent. 2014;35(5):344. 348.
Levrini L, Di Benedetto G, Raspanti M. Dental wear: a scanning electron microscope study. Biomed Res Int. 2014;2014:340425.
Donaldson LF. Understanding pulpitis. J Physiol. 2006;573(Pt 1):2–3.
Tripodi D, Latrofa M, D'Ercole S. Microbiological aspects and inflammatory response of pulp tissue in traumatic dental lesions. Eur J Inflamm. 2007;5(3):115–119.
Carda C, Peydró A. Ultrastructural patterns of human dentinal tubules, odontoblasts processes and nerve fibres. Tissue Cell. 2006;38(2):141–150.
Lo Giudice G, Cutroneo G, Centofanti A, et al. Dentin morphology of root canal surface: a quantitative evaluation based on a scanning electronic microscopy study. Biomed Res Int. 2015;2015:164065.
Nalla RK, Porter AE, Daraio C, et al. Ultrastructural examination of dentin using focused ion-beam crosssectioning and transmission electron microscopy. Micron. 2005;36(7–8):672–680.
Lopes MB, Sinhoreti MA, Gonini Júnior A, Consani S, McCabe JF. Comparative study of tubular diameter and quantity forhuman and bovine dentin at different depths. Braz Dent J. 2009;20(4):279–283.
Ghazali FB. Permeability of dentine. Malays J Med Sci. 2003;10(1):27–36.
Ciocca L, Gallina I, Navacchia E, Baldissara P, Scotti R. A new method for quantitative analysis of dentinal tubules. Comput Biol Med. 2007;37(3):277–286.
Agematsu H, Sawada T, Watanabe H, Yanagisawa T, Ide Y. Immuno-scanning electron microscope characterization of large tubules in human deciduous dentin. Anat Rec. 1997;248(3):339–345.
Tafforeau TM, Smith P. New visions of dental tissue research: tooth, development, chemistry, and structure. Evol Anthrop. 2008;17(2):213–226.
Journals System - logo
Scroll to top