Increased TNF-α and TGF-β concentrations in rat liver after intense exercise
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Department of Clinical Nursing, Medical University of Warsaw, Warsaw, Poland
Department of Immunology, Transplantology, and Internal Diseases, Medical University of Warsaw, Warsaw, Poland
Department of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, Bialystok, Poland
Bożena Czarkowska-Pączek   

Department of Clinical Nursing, Medical University of Warsaw, E. Ciolka 27, 01-445 Warsaw, Poland. Tel./fax: +4822 836 09 72.
Submission date: 2017-09-06
Acceptance date: 2017-11-27
Online publication date: 2018-02-02
Publication date: 2019-11-17
Pol. Ann. Med. 2018;25(1):98–102
Intense exercise can cause cellular damage, resulting in activation of inflammatory and fibrotic reactions.

The aim of the present study was to investigate how one session of intense exercise affected liver gene expression and concentrations of tumor necrosis factor α (TNF-α) and profibrotic transforming growth factor β (TGF-β) in untrained and trained rats.

Material and methods:
Here we investigated the impact of intense exercise on liver gene expression and concentrations of TNF-α and TGF-β in untrained and trained healthy rats (n = 30 each). The trained rats underwent 6 weeks of endurance training with increasing load. From each group, liver samples were collected before, immediately after, and 3 h after one session of intense exercise. Gene expression was evaluated with quantitative real-time polymerase chain reaction. Protein content was measured by enzyme immunoassay.

Results and discussion:
One session of intense exercise did not influence gene expression at any time point. In trained rats TNF-α and TGF-β was increased immediately after exercise (P = 0.011 and P = 0.009, respectively). The increase in TGF-β persisted 3 h post exercise (P = 0.045). In untrained rats the concentration of TNF-α did not change in any time point, while TGF-β was decreased both immediately and 3 h after intense exercise (P = 0.01 and P = 0.03, respectively).

Intense exercise led to increased proinflammatory and profibrotic activity in the liver of trained rats.

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
The authors declare that there is no conflict of interest.
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