Lack of association between the S1082 (A/G) IL-10 polymorphism (rs1800896) and spontaneous preterm birth in the Indonesian Acehnese population
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Department of Obstetrics and Gynecology, School of Medicine, Syiah Kuala University, Banda Aceh, Indonesia
Department of Obstetrics and Gynecology, Dr. Zainoel Abidin Hospital, Banda Aceh, Indonesia
Department of Obstetrics and Gynaecology, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia
Department of Pharmacology, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia
Medical Research Unit, School of Medicine, Syiah Kuala University, Banda Aceh, Indonesia
Department of Microbiology, School of Medicine, Syiah Kuala University, Banda Aceh, Indonesia
Submission date: 2016-10-13
Acceptance date: 2016-11-21
Online publication date: 2016-12-08
Publication date: 2019-12-20
Corresponding author
Mohd Andalas   

Medical Research Unit, Department of Obstetrics and Gynecology, School of Medicine, Syiah Kuala University, Jl. T. Tanoeh Abe, Darussalam, Banda Aceh 23111, Indonesia. Tel.: +62 0651 7551843; fax: +62 0651 7551843.
Pol. Ann. Med. 2017;24(2):209-213
Preterm birth is associated with multiple mechanisms, and a contractile state during preterm birth is typically accompanied by a shift in signaling from anti-inflammatory to pro-inflammatory pathways. It has been hypothesized that a mutation within the promoter of the interleukin-10 gene (IL-10) that causes hypo- or hyper-production of IL-10 might be associated with spontaneous preterm birth.

To determine the association between the –1082 (G/A) single nucleotide polymorphism (SNP) of IL-10 and spontaneous preterm birth in Aceh, Indonesia.

Material and methods:
A case-control study was conducted between June 2012 and July 2014 at Dr. Zainoel Abidin Hospital, Banda Aceh. A total of 40 preterm and 40 term births were included in the final analysis. Genotyping of the –1082 (G/A) IL-10 SNP was conducted using real-time polymerase chain reaction (RT-PCR) and was confirmed by sequencing. An enzyme-linked immunosorbent assay (ELISA) was performed to measure the level of IL-10 in sera. The associations of the genotype distribution and allele frequency with IL-10 levels and preterm birth were assessed using the χ2 test.

Results and discussion:
There was no association between the genotype distribution or allele frequency and the level of IL-10 in serum. There was also no association between the genotype distribution or allele frequency and spontaneous preterm birth.

There is no strong association between the –1082 (A/G) IL-10 SNP and spontaneous preterm birth in the Acehnese ethnic group.

We would like to thank Prodia Laboratory Banda Aceh, which provided services related to the collection, processing, transport and storage of serum samples, and Prodia Laboratory Jakarta for genotyping.
None declared.
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