RESEARCH PAPER
Lack of association between the S1082 (A/G) IL-10 polymorphism (rs1800896) and spontaneous preterm birth in the Indonesian Acehnese population
Mohd Andalas 1, 2  
,   Mohammad Hakimi 3,   Detty S Nurdiati 3,   Indwiani Astuti 4,   Ichsan Ichsan 5, 6,   Nur Wahyuniati 5,   Imran Imran 5,   Harapan Harapan 5, 6  
 
More details
Hide details
1
Department of Obstetrics and Gynecology, School of Medicine, Syiah Kuala University, Banda Aceh, Indonesia
2
Department of Obstetrics and Gynecology, Dr. Zainoel Abidin Hospital, Banda Aceh, Indonesia
3
Department of Obstetrics and Gynaecology, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia
4
Department of Pharmacology, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia
5
Medical Research Unit, School of Medicine, Syiah Kuala University, Banda Aceh, Indonesia
6
Department of Microbiology, School of Medicine, Syiah Kuala University, Banda Aceh, Indonesia
CORRESPONDING AUTHOR
Mohd Andalas   

Medical Research Unit, Department of Obstetrics and Gynecology, School of Medicine, Syiah Kuala University, Jl. T. Tanoeh Abe, Darussalam, Banda Aceh 23111, Indonesia. Tel.: +62 0651 7551843; fax: +62 0651 7551843.
Submission date: 2016-10-13
Acceptance date: 2016-11-21
Online publication date: 2016-12-08
Publication date: 2019-12-20
 
Pol. Ann. Med. 2017;24(2):209–213
 
KEYWORDS
ABSTRACT
Introduction:
Preterm birth is associated with multiple mechanisms, and a contractile state during preterm birth is typically accompanied by a shift in signaling from anti-inflammatory to pro-inflammatory pathways. It has been hypothesized that a mutation within the promoter of the interleukin-10 gene (IL-10) that causes hypo- or hyper-production of IL-10 might be associated with spontaneous preterm birth.

Aim:
To determine the association between the –1082 (G/A) single nucleotide polymorphism (SNP) of IL-10 and spontaneous preterm birth in Aceh, Indonesia.

Material and methods:
A case-control study was conducted between June 2012 and July 2014 at Dr. Zainoel Abidin Hospital, Banda Aceh. A total of 40 preterm and 40 term births were included in the final analysis. Genotyping of the –1082 (G/A) IL-10 SNP was conducted using real-time polymerase chain reaction (RT-PCR) and was confirmed by sequencing. An enzyme-linked immunosorbent assay (ELISA) was performed to measure the level of IL-10 in sera. The associations of the genotype distribution and allele frequency with IL-10 levels and preterm birth were assessed using the χ2 test.

Results and discussion:
There was no association between the genotype distribution or allele frequency and the level of IL-10 in serum. There was also no association between the genotype distribution or allele frequency and spontaneous preterm birth.

Conclusions:
There is no strong association between the –1082 (A/G) IL-10 SNP and spontaneous preterm birth in the Acehnese ethnic group.

ACKNOWLEDGEMENTS
We would like to thank Prodia Laboratory Banda Aceh, which provided services related to the collection, processing, transport and storage of serum samples, and Prodia Laboratory Jakarta for genotyping.
CONFLICT OF INTEREST
None declared.
 
REFERENCES (25)
1.
Blencowe H, Cousens S, Chou D, et al. Born too soon: the global epidemiology of 15 million preterm births. Reprod Health. 2013;10(suppl 1):S2.
 
2.
Hodek JM, von der Schulenburg JM, Mittendorf T. Measuring economic consequences of preterm birth – methodological recommendations for the evaluation of personal burden on children and their caregivers. Health Econ Rev. 2011;1(1):6.
 
3.
Ryan JG, Dogbey E. Preterm births: a global health problem. MCN Am J Matern Child Nurs. 2015;40(5):278–283.
 
4.
Lawn JE, Gravett MG, Nunes TM, Rubens CE, Stanton C. Global report on preterm birth and stillbirth (1 of 7): definitions, description of the burden and opportunities to improve data. BMC Pregnancy Childbirth. 2010;10(suppl 1):S1.
 
5.
Goldenberg RL, Culhane JF, Iams JD, Romero R. Epidemiology and causes of preterm birth. Lancet. 2008;371(9606):75–84.
 
6.
Menon R. Spontaneous preterm birth, a clinical dilemma: etiologic, pathophysiologic and genetic heterogeneities and racial disparity. Acta Obstet Gynecol Scand. 2008;87(6):590–600.
 
7.
Goldenberg RL, Hauth JC, Andrews WW. Intrauterine infection and preterm delivery. N Engl J Med. 2000;342(20):1500–1507.
 
8.
Agrawal V, Hirsch E. Intrauterine infection and preterm labor. Semin Fetal Neonatal Med. 2012;17(1):12–19.
 
9.
Kemp MW. Preterm birth, intrauterine infection, and fetal inflammation. Front Immunol. 2014;5:574.
 
10.
Menon R, Fortunato SJ. Infection and the role of inflammation in preterm premature rupture of the membranes. Best Pract Res Clin Obstet Gynaecol. 2007;21(3):467–478.
 
11.
Romero R, Espinoza J, Kusanovic JP, et al. The preterm parturition syndrome. BJOG. 2006;113(suppl 3):17–42.
 
12.
Gotsch F, Romero R, Erez O, et al. The preterm parturitionsyndrome and its implications for understanding the biology, risk assessment, diagnosis, treatment and prevention of preterm birth. J Matern Fetal Neonatal Med. 2009;22(suppl 2):5–23.
 
13.
Moore KW, de Waal Malefyt R, Coffman RL, O'Garra A. Interleukin-10 and the interleukin-10 receptor. Annu Rev Immunol. 2001;19:683–765.
 
14.
Tso HW, Ip WK, Chong WP, Tam CM, Chiang AK, Lau YL. Association of interferon gamma and interleukin 10 genes with tuberculosis in Hong Kong Chinese. Genes Immun. 2005;6(4):358–363.
 
15.
Blanco-Quirós A, Arranz E, Solis G, Villar A, Ramos A, Coto D. Cord blood interleukin-10 levels are increased in preterm newborns. Eur J Pediatr. 2000;159(6):420–423.
 
16.
Barrilleaux PS, Rodts-Palenik S, Terrone D, et al. Combined antibiotic/interleukin-10 therapy increases interval to delivery in a rat model of infection-mediated preterm birth. Am J Obstet Gynecol. 2001;185(6):S87.
 
17.
Wommack JC, Ruiz RJ, Marti CN, Stowe RP, Brown CE, Murphey C. Interleukin-10 predicts preterm birth in acculturated Hispanics. Biol Res Nurs. 2013;15(1):78–85.
 
18.
Andalas M, Hakimi M, Nurdiati D, Astuti I, Imran I, Harapan H. Association of 308G/A TNF-a gene polymorphism and spontaneous preterm birth in Acehnese ethnic group, Indonesia: this polymorphism is not associated with preterm birth. Egypt J Med Hum Genet. 2016;17(1):33–40.
 
19.
Moura E, Mattar R, de Souza E, Torloni MR, Gonçalves-Primo A, Daher S. Inflammatory cytokine gene polymorphisms and spontaneous preterm birth. J Reprod Immunol. 2009;80(1–2): 115–121.
 
20.
Ohashi J, Naka I, Patarapotikul J, Hananantachai H, Looareesuwan S, Tokunaga K. Lack of association between interleukin-10 gene promoter polymorphism, 1082G/A, and severe malaria in Thailand. Southeast Asian J Trop Med Public Health. 2002;33(suppl 3):5–7.
 
21.
Syukri M, Imran M, Harapan H, Sja'bani M, Soesatyo MHN, Astuti I. There is no correlation between the functional polymorphism 460C>T in vascular endothelial growth factor (VEGF) gene promoter and uncomplicated recurrent urinary tract infection among young women. Pol Ann Med. 2015;22(1):5–10.
 
22.
Imran I, Lamsudin R, Idjradinata P, et al. Association of betafibrinogen promoter gene polymorphism (–148C/T), hyperfibrinogenemia and ischemic stroke in young adult patients. Egypt J Med Hum Genet. 2015;16(1):11–17.
 
23.
Zhu Q, Sun J, Chen Y. Preterm birth and single nucleotide polymorphisms in cytokine genes. Transl Pediatr. 2014;3(2):120–134.
 
24.
Suki SZ, Omar SZ, Mohamed Z. Association study of interleukin 10 1082 G/A polymorphism and interleukin-10 levels with occurrence of spontaneous preterm birth in a triethnic Malaysian population. Public Health Genomics. 2015;18:27.
 
25.
Turner DM, Williams DM, Sankaran D, Lazarus M, Sinnott PJ, Hutchinson IV. An investigation of polymorphism in the interleukin-10 gene promoter. Eur J Immunogenet. 1997;24(1):1–8.