New method in assessment of blood plasma coagulation balance
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Department of Physical Education and Physical Therapy, National Pirogov Memorial Medical University, Vinnytsia, Ukraine
Research Institute of Rehabilitation of National Pirogov Memorial Medical University, Vinnytsya, Ukraine
Department of Internal Medicine No 3, National Pirogov Memorial Medical University, Vinnytsia, Ukraine
Vinnytsia Regional Center of Medical and Social Examination, Vinnytsia, Ukraine
Submission date: 2021-02-18
Final revision date: 2021-03-30
Acceptance date: 2021-03-30
Online publication date: 2021-10-20
Corresponding author
Borys Storozhuk   

Department of Internal Medicine No 3, National Pirogov Memorial Medical University, 56 Street Pirogova, Vinnytsia, Ukraine. Tel.: +38 050-313-3302
Pol. Ann. Med. 2022;29(2):94-98
Thrombotic complications associated with disorders of hemostatic system are quite common among various categories of patients and require timely objective assessment of coagulation balance.

To develop an integrated index of assessment of blood plasma coagulation balance using the method of ‘overall haemostasis potential,’ and to test its practical value in clinical settings.

Material and methods:
179 patients were studied: 88 with chronic kidney disease (CKD) of VD stage, treated by program hemodialysis, and 91 with ischemic heart disease (IHD) after percutaneous transluminal coronary angioplasty (PTСA). The state of overall coagulation and fibrinolytic potentials was studied in all patients using the method of overall hemostasis potential (OHP). Based on this method, coagulation index (CI) was calculated and tested in such categories of patients.

Results and discussion:
Patients with stage VD CKD demonstrated the tendency to hypocoagulation, while those with coronary heart disease (CHD) and PTСA – significant hypercoagulation, being indicated by CI index.

Patients with stage VD CKD have the tendency to hypocoagulation, while those with CHD and PTСA – to hypercoagulation. The integrated index suggested for assessment of coagulation potential – coagulation index – can adequately reflect coagulation balance disorders and considerably simplify their quantitative assessment.

The study was conducted as a part of research work ‘To determine the role of pro- and anticoagulant factors of hemostasis and coagulation potential parameters in the development of comorbid conditions in patients with chronic kidney disease VD stage and to develop the criteria for thrombophilia prevention,’ financed by the Ministry of Health of Ukraine at the state budget expense, state registration number 0119U101156.
The authors declare no conflict of interest.
Rublenko AM, Urvant LP, Makogonenko YeM, et al. Influence of protein C activator on overall hemostasis potential of blood plasma in hip arthroplasty [in Ukrainian]. Ukr Biokhim Zh. 2011;83(5):32–38.
He S, Blomback M, Wallen H, Jeppson A, Grass S. Global impairments in the hаemostasis systems after cardiopulmonary bypass. Thrombosis Research. 2017;151:63–66.
Volkov GL, Platonova TN, Savchuk AM, Gornitskaya OV, Chernyshenko TM, Krasnobryzha YeM. Modern Ideas on Hemostasis System. Kiev: Naukova Dumka; 2005.
Scandura JM, Walsh PN. Factor X bound to the surface of activated human platelets is preferentially activated by platelet-bound factor IXa. Biochemistry. 1996;35(27):8903–8913.
Platonova TN, Zaichko NV, Chernyshenko TM, Gornitskaya OV, Grishchuk VI. Assessment of informative value and prognostic significance of traditional screening and additional laboratory tests for thrombophilia diagnosis [in Russian]. Lab Diagnostics. 2010;4(54):3–10.
Amosova EN. Guide on Thrombolytic Therapy [in Ukrainian]. Kiev: IT-studio; 1998.
Blombäck B. Fibrinogen and fibrin – proteins with complex roles in haemostasis and thrombosis. Thromb Res. 1996;83(1):1–75.
Pyrogova LV, Chernyshenko TM, Kolesnikova IN, Platonova TN, Bereznitsky GK, Makogonenko YeM et al. Level of overall haemostasis potential in donor and patient plasma in pathology. Ukr Biochem J. 2016;88(2):56–65.
Meh DA, Siebenlist KR, Mosesson MW. Identification and characterization of the thrombin binding sites on fibrin. J Biol Chem. 1996;271(38):23121–23125.
Meh DA, Siebenlist KR, Brenann SO, Holyst T, Mosesson MW. The amino acid sequence in fibrin responsible for high affinity thrombin binding. Thromb Hemost. 2001;85(3):470–474.
Di Cera EJ. Thrombin as procoagulant and anticoagulant. Thromb Hemost. 2007;5(1):196–202.
Storozhuk NV, Storozhuk BG, Makogonenko YeM, Storozhuk LO, Pirogova LV, Storozhuk OB et al., inventors. Research Institute of Rehabilitation of Individuals with Disabilities, Vinnytsia National Pirogov Memorial Medical University, patent owner. The method of determining blood plasma coagulation balance. Patent of Ukraine No 122516. 2020.11.26.
Melnik АА. System of hemostasis and its regulation in renal dysfunction [in Russian]. News Medicine and Pharmacy in Ukraine. 2016;9(583):24–31.
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