Serum isoniazid concentration in the patients as an indicator of the effectiveness and toxicity of tuberculosis treatment
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Department of General and Clinical Pharmacology, Odessa National Medical University, Ukraine
Department of Biotechnology and Molecular Biology, Opole University, Poland
Petro B. Antonenko   

Odessa National Medical University, Valichovsky lane, 2, Department of General and Clinical Pharmacology, 65023 Odessa, Ukraine. Tel.: +38 0975875636.
Submission date: 2018-09-24
Final revision date: 2018-11-28
Acceptance date: 2018-11-29
Online publication date: 2019-12-11
Pol. Ann. Med. 2019;26(2):139–144
The low serum concentration of the anti-tuberculosis (TB) drugs in TB patients is observed quite often, but the significance of this phenomenon remains controversial.

The aim of the presented research was to clarify the association between isoniazid concentration and TB-treatment outcomes.

Material and methods:
Blood samples were obtained from 86 patients with newly diagnosed pulmonary TB at Odessa Regional TB Hospital in 2015. The level of isoniazid in blood was measured 2 h, 4 h, 6 h, and 24 h after the administration of isoniazid. The medical records of the enrolled patients at the beginning and at the end of the in-patient treatment, and the activity of the biochemical indices were considered.

Results and discussion:
At the end of the in-patient treatment in patients with a serum isoniazid concentration of more than 2 µg/mL (HIC), 4 h after the drug administration, the resorption of TB infiltrates in lungs occurred 1.3 times more frequently than in patients with an isoniazid concentration of less than 2 µg/mL (LIC). According to the culture method, the smear conversion occurred 3.1 times more often in patients with HIC than in the LIC ones. A positive correlation was found between the isoniazid serum level with the bilirubin level, thymol probe, and the AsAT activity, which proved the higher risk of hepatotoxicity in patients with HIC.

Thus, the measurement of the isoniazid concentration after 4 h of isoniazid administration can predict the outcome of the TB-treatment. It is postulated that the recommended concentration of isoniazid in blood 4 h after its administration > 2 µg/mL.

The authors declare no conflict of interests.
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