RESEARCH PAPER
The effect of sodium selenate on biochemical and morphological parameters of blood and reproductive indicators of sheep of selected breeds used in the Czech Republic
Jan Micinski 1  
,   Radek Filipčík 2  
,   Jan Kuchtik 2  
,   Miroslav Marsalek 2  
,   Tomas Kopec 2  
 
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1
Department of Sheep and Goat Breeding, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Poland
2
Department of Animal Breeding, Faculty of Agriculture, Mendel University in Brno, Brno, czech Republic
CORRESPONDING AUTHOR
Jan Micinski   

Department of Sheep and Goat Breeding, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5/150, 10-719 Olsztyn, Poland.
Submission date: 2021-08-04
Final revision date: 2021-08-26
Acceptance date: 2021-08-26
Online publication date: 2021-10-01
 
Pol. Ann. Med. 2021;28(2):199–205
 
KEYWORDS
TOPICS
ABSTRACT
Introduction:
In the Czech Republic, there is a deficiency of selenium in sheep due to its low level of occurrence in soil, thus in fodder. Selenium deficiency in these animals can manifest in a number of diseases. Therefore, sheep should be supplemented with preparations containing selenium with vitamin E, because Se is closely related to vitamin E, which plays an important role in the immune system.

Aim:
The aim of the study was to assess the selenium status of selected sheep breeds in the Czech Republic and to determine the effect of selenium and vitamin E supplementation on selected hematological, biochemical and enzymatic blood indicators and on reproductive indicators.

Material and methods:
The research material consisted of sheep, used in the Czech Republic, of three breeds: Šumavka (16), Valaszka (16) and Zwartbles (16). At the 3rd month of gestation, the sheep were injected with sodium selenate with vitamin E. In the blood of sheep, hematological, biochemical and enzymatic parameters were analized, and reproductive indicators of sheep were determined.

Results and discussion:
The concentration of Se in sheep was quite low at the beginning of the experiment (from 0.145 (AVR group) to 0.219 µmol/l (BZR group) and the highest concentration (1.322 µmol/l I BZR group) on the test III. It was shown in experimental groups that with increasing selenium concentration, GSH-Px activity increased from the lowest level of 53.77 (ASR group) to the highes: 222.88 U/gHb (BZR group). In sheep from experimental groups (ASR, AVR, BZR groups) better fertility was obtained, amounting to 96.3%, 95.5%,89.4%, respectively.

Conclusions:
In all groups of sheep there was a selenium deficiency. Supplementation turned out to be an effective method of compensating for its deficiency. The proper selenium status ensured better reproductive indicators for sheep.

ACKNOWLEDGEMENTS
Thank you very much prof. Radkovi Filipcikovi, head of the Department of Animal Breeding at the University of G. Mendel in Brno together with employees for enabling the internship and providing research material.
FUNDING
Project financially supported by Minister of Science and Higher Education in the range of the program entitled ‘Regional Initiative of Excellence’ for the years 2019–2022, Project No. 010/RID/2018/19, amount of funding 12 000 000 PLN.
CONFLICT OF INTEREST
Authors have no conflict of interest.
 
REFERENCES (33)
1.
CzSO (Czech Statistical Office). 2020. https://www.czso.cz/csu/czso/h....
 
2.
CSO (Central Statistical Office). https://stat.gov.pl/obszary-te....
 
3.
PSO (Polish Sheep Association). Sheep and goat breeding in Poland in 2019 year. Warsaw: PSO; 2020:1–71. http://www.pzow.pl.
 
4.
SChOK (Svaz chovatelú ovci a koz z.s.). Report. Assessment of the results of sheep and goat breeding in the Czech Republic for 2019. Praha: Praga. 2020. https://new.schok.cz.
 
5.
Pogorzelska J, Miciński J, Ostoja H, Kowalski IM, Szarek J, Strzyżewska J. Quality traits of meat from young limousin, charolais and hereford bulls. Pak Vet Sci Res. 2013;33(1): 65–68.
 
6.
Dzik S, Miciński B, Miciński J, Mituniewicz T, Kowalski PM. Therapeutic properties of lactoferrin. Pol Ann Med. 2018;25(1):158–161. https://doi.org/10.29089/2017.....
 
7.
Miciński J, Zwierzchowski G, Kowalski IM, Szarek J, Pierożyński B, Raistenskis J. The effects of bovine milk fat on human health. Pol Ann Med. 2012;19(2):170–175. https://doi.org/10.1016/j.poam....
 
8.
Miciński J, Zwierzchowski G, Kowalski IM, Wojtkiewicz J, Szarek J. Health-supporting properties of beef. J Elem. 2012;17(1):149–157. https://doi.org/10.5601/jelem.....
 
9.
Miciński J, Zwierzchowski G, Kowalski IM, Szarek J. Health – promoting properties of selected milk components. J Elem. 2013;18(1):165–186. https://doi.org/10.5601/jelem.....
 
10.
Pilarczyk B, Balicka-Ramisz A, Ramisz A, Pilarczyk R, Sablik P, Wójcik J. Blood serum levels of selenium in cows and heifers imported to Poland from Germany, the Czech Republic, France and the Netherlands. Tierärztl Umschau. 2008;63(5):243–246.
 
11.
Underwood EJ, Suttle NF. The mineral nutrition of livestock. 3rd Ed. New York: Cabi Publ. 1999:421–475.
 
12.
Kamada H, Nonaka I, Ueda Y, Murai M. Selenium addition to colostrum increases immunoglobulin G absorption by newborn calves. J Dairy Sci. 2007;90(12):5665–5670. https://doi.org/10.3168/jds.20....
 
13.
Qin S, Gao J, Huang K. Effects of different selenium sources on tissue selenium concentrations, blood GSH-Px activities and plasma interleukin levels in finishing lambs. Biol Trace Elem Res. 2007;116(1):91–102. https://doi.org/10.1007/bf0268....
 
14.
Koenig KM, Rode LM, Cohen RD, Buckley WT. Effects of diet and chemical form of selenium on selenium metabolism in sheep. J Anim Sci. 1997;75(3):817–827. https://doi.org/10.2527/1997.7....
 
15.
Lenartova V, Holovska K, Javorsky P. The influence on the antioxidant enzyme activity of rumen bacteria Streptococcus bovis and Selenomonas ruminantium. FEMS Microbiol Ecol. 1998;27(4):319–325. https://doi.org/10.1111/j.1574....
 
16.
Osame S, Takahashi H, Furuoka H, Matsui T, Ichijo S. Clinicopathological observations on white muscle disease in goat kids. J Jap Vet Med Ass. 1994;47:474–477.
 
17.
Barham D, Trinder P. An improved colour reagent for the determination of blood glucose by the oxidase system. Analyst. 1972;97(151):142–145. https://doi.org/10.1039/an9729....
 
18.
Lie RF, Schurtz JM, Kenneth JP, Gochonan N. Cholesterol oxidase-based determination by continuous-flow analysis of total and free cholesterol in serum. Clin Chem. 1976;22(10):1627–1630.
 
19.
Mc Gowan MW, Artiss JD, Stranbergh DR, Zak BA. Peroxidase-coupled method for colorimetric determination of serum triglycerides. Clin Chem. 1983;29(3):538–542.
 
20.
Huang X, Chai J, Im H, Yarimaga O, Yoon E, Kim H. Aspartate aminotransferase (AST, GOT) and alanine aminotransferase (ALT, GPT) detection techniques. Sensors. 2006;6(7):756–782. https://doi.org/10.3390/s60707....
 
21.
Burtis CA, Ashwood ER. Tietz Textbook of Clinical Chemistry. 2nd Ed. Philadelphia, USA: WB Saunders; 1994:816–818.
 
22.
StatSoft, I. Electronic Statistics Textbook. 2018. www.statsoft.pl/textbook/stath.... Accessed: 20.08.2021.
 
23.
Sharma DK, Chauhan PPS, Saxena VK, Agrawal RD. Haematological changes in experimental trypanosomasis in Barbari goats. Small Rumin Res. 2000;38:145–149. https://doi.org/10.1016/S0921-....
 
24.
Mueller AS, Palluf J, Rafael J. The chemical form of selenium affects insulinometric properties of the trace element: investigations in type II diabetic dbdb mice. J Nutr Biochem. 2003;14(11):637–647. https://doi.org/10.1016/j.jnut....
 
25.
Brzezińska M, Orowicz W, Pieczyńska M. Content of some macroelements in blood serum and hematological parameters in goats of different age. J Elem. 2005;10(S):22–23.
 
26.
Pavlata L, Podhorsky A, Pechova A, Dvorak R. Incidence of hypovitaminosis E in calves and therapeutic remedy by Selenium-vitamin supplementation. Acta Vet Brno. 2005;74:209–216. https://doi.org/10.2754/avb200....
 
27.
Caox Y, Maddox JF, Mastro AM, Scholz RW, Hildebrandt G, Reddy CC. Selenium deficiency alters the lipooxygenase pathway and mitogenic response in bovine lymphocytes. J Nutr. 1992;122(11):2121–2127. https://doi.org/10.1093/jn/122....
 
28.
Ramirez-Bribiesca JE, Tortora JL, Huerta M. Diagnosis of selenium status in grazing dairy goats on the Mexican plateau. Small Rumin Res. 2001;41(1):81–85. https://doi.org/10.1016/s0921-....
 
29.
Bickhardt K, Ganter M, Sallmann P, Fuhrmann H. [Investigations on manifestations of vitamin E and selenium deficiency in sheep and goats]. Deut Tierar Wochen. 1999;106(6):242–247 [in German].
 
30.
Hudman JF, Costa ND, Robinson WF. An apparent phosphate selenium interaction in weaner sheep. J Trace Elem Electr Health Dis. 1988;2(2):105–109.
 
31.
Pavlata L, Pechova A, Illek J. Direct and indirect assesment of selenium status in cattle – a comparison. Acta Vet Brno. 2000;69:281–287. https://doi.org/10.2754/avb200....
 
32.
Sobiech P, Platt-Samoraj A, Michalski M. Activity of LDH isoenzymes in diarrhea in kids goats [in Polish]. Med Wet. 2005;61:100–102.
 
33.
Pavlata L, Pechova A, Illek J. Muscular dystrophy in dairy cows following a change in housing technology. Acta Vet Brno. 2001;70:269–275. https://doi.org/10.2754/avb200....