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RESEARCH PAPER
The expression of Bax protein in the early stages of spinal cord injury in the sperm cells of rats
| 1 | Department of Anatomy and Neuroscience, Shahrekord University of Medical Sciences, Shahrekord, Iran |
| 2 | Department of Surgery, Faculty of Medicine, Kurdistan University of Medical Sciences, Kurdistan, Iran |
| 3 | Department of Anatomy and Reproductive Biology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran |
| 4 | Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran |
| 5 | Deputy of Research, Kurdistan University of Medical Sciences, Sanandaj, Iran |
| 6 | Cellular and Molecular Research Center, Department of Anatomical Sciences, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran |
CORRESPONDING AUTHOR
Mohammad Jafar Rezaie
Cellular and Molecular Research Center, Department of Anatomical Sciences, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran. Tel.: +988733664653, Fax: +988716664663.
Cellular and Molecular Research Center, Department of Anatomical Sciences, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran. Tel.: +988733664653, Fax: +988716664663.
Submission date: 2017-04-19
Acceptance date: 2017-07-11
Online publication date: 2018-06-18
Publication date: 2019-11-18
Pol. Ann. Med. 2018;25(2):196–202
KEYWORDS
ABSTRACT
Introduction:
Apoptosis is one of the most important biological processes, which occurs through the activation of intracellular cell death pathway.
Aim:
The aim of this study was to determine the pattern of cell death within the early stages of spinal cord injury (SCI) and the evaluation of the changes in Bax protein expression of progressive apoptosis.
Material and methods:
48 adult male Sprague Dawley rats were randomly divided into two control and experimental groups. Animals were anesthetized and then the laminectomy procedure was performed in the area of T6–8. On days 1, 7, 14 and 28 after the surgery, one-third of the middle part of their testis tissue were taken for histological and immunohistochemical analysis.
Results:
The immunohistochemical analysis indicated the presence of TUNEL-positive cells and cells containing the pro-apoptotic protein Bax in testicular sperms in the 1st day after SCI, as well as increased on 28 days.
Discussion:
1 day after SCI, the apoptosis occurred in testicular sperm lines as well as the apoptosis can be related to Bax protein expression. Therefore inhibition of caspase activity via caspase cascades-mediated apoptosis may have a protective role in testicular injury during the acute phase of SCI.
Conclusions:
Our finding suggested that the damage and destruction after SCI can be controlled by therapeutic interventions at the appropriate time before the destructive changes of SCI.
Apoptosis is one of the most important biological processes, which occurs through the activation of intracellular cell death pathway.
Aim:
The aim of this study was to determine the pattern of cell death within the early stages of spinal cord injury (SCI) and the evaluation of the changes in Bax protein expression of progressive apoptosis.
Material and methods:
48 adult male Sprague Dawley rats were randomly divided into two control and experimental groups. Animals were anesthetized and then the laminectomy procedure was performed in the area of T6–8. On days 1, 7, 14 and 28 after the surgery, one-third of the middle part of their testis tissue were taken for histological and immunohistochemical analysis.
Results:
The immunohistochemical analysis indicated the presence of TUNEL-positive cells and cells containing the pro-apoptotic protein Bax in testicular sperms in the 1st day after SCI, as well as increased on 28 days.
Discussion:
1 day after SCI, the apoptosis occurred in testicular sperm lines as well as the apoptosis can be related to Bax protein expression. Therefore inhibition of caspase activity via caspase cascades-mediated apoptosis may have a protective role in testicular injury during the acute phase of SCI.
Conclusions:
Our finding suggested that the damage and destruction after SCI can be controlled by therapeutic interventions at the appropriate time before the destructive changes of SCI.
ACKNOWLEDGEMENTS
Authors would like to thank personnel of deputy of research and lab staffs in Kurdistan University of Medical Sciences, Sanandaj, Iran for their technical and assistant support.
CONFLICT OF INTEREST
The authors declare no conflict of interest.
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