Usefulness of clinical magnetic resonance scanners for imaging experimental changes in laboratory rodents’ central nervous system
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Department of Neurology and Neurosurgery, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Poland
Department of Nuclear Medicine and Endocrine Oncology, The Maria Skłodowska-Curie Memorial Cancer Center, Institute of Oncology, Gliwice Branch, Poland
Dorota Mystkowska   

Stem Cells Laboratory, Faculty of Medical Sciences, University of Warmia and Mazury, Warszawska 30, 10-082 Olsztyn, Poland. Tel.: +4889 524 53 38; fax: +4889 524 53 18.
Submission date: 2011-10-12
Acceptance date: 2012-01-21
Publication date: 2020-04-10
Pol. Ann. Med. 2012;19(1):43–49
Magnetic resonance imaging (MRI) is a noninvasive technique applied in medical diagnosis and for studying animal models of human diseases. MRI offers longitudinal in vivo studies without the need to sacrifice animals, thus making data easier to compare. The number of required animals can be limited.

The aim of this article was to present the potential role of clinical MRI scanners in the management of central nervous system visualization and injury in rodents on the basis of the current literature.

Material and methods:
Clinical small bore scanners with field strength from 0.1 T to 3 T are used for imaging the nervous system of rodents in vivo.

Results and discussion:
The employment of clinical scanners equipped with dedicated human coils, for small objects imaging, results in the reduction of image quality. It is caused by a small signal-to-noise ratio (SNR). The way to increase the SNR is to use clinical scanners for imaging particular parts of the human body, e.g. head, or dedicated coils for imaging small parts of the human body, e.g., thumb or wrist, or to use dedicated small animal coils to image multiple animals in the larger bore of the clinical scanner at the same time. For some neurobiological experiments clinical scanners seem to be sufficient. Although clinical MRI scanners are widespread, not many laboratories use them for small animal research.

Clinical scanners with surface coils dedicated to small human organs, or with dedicated small animal coils, are useful for imaging experimental changes in the central nervous system of laboratory rodents.

None declared.
Bladowska J, Sokolska V, Czapiga E, Badowski R, Koźmińska U, Moroń K. Advances in diagnostic imaging of the pituitary and the parasellar region. Adv Clin Exp Med. 2004;13(4):709–717.
Bobek-Billewicz B, Stasik-Pres G, Majchrzak H, Zarudzki Ł. Differentiation between brain tumor recurrence and radiation injury using perfusion, diffusion-weighted imaging and MR spectroscopy. Folia Neuropathol. 2010;48(2):81–92.
Brekke C, Lundervold A, Enger PØ, Brekken C, Stålset E, Pedersen TB, et al. NG2 expression regulates vascular morphology and function in human brain tumours. Neuroimage. 2006;29(3):965–976.
Breton E, Goetz C, Choquet P, Constantinesco A. Low field magnetic resonance imaging in rat in vivo. IRBM 2008;29(6):366–374.
Brockmann MA, Ulmer S, Leppert J, Nadrowitz R, Wuestenberg R, Nolte I, et al. Analysis of mouse brain using a clinical 1.5 T scanner and standard small loop surface coil. Brain Res. 2006;1068(1):138–142.
Brockmann MA, Kemmling A, Groden C. Current issues and perspectives in small rodent magnetic resonance imaging using clinical MRI scanners. Methods 2007;43(1):79–87.
Chang NK, Jeong YY, Park JS, Jeong HS, Jang MJ, Lee JH, et al. Tracking of neural stem cells in rats with intracerebral hemorrhage by the use of 3 T MRI. Korean J Radiol. 2008;9(3):196–204.
Engelhorn T, Eyupoglu IY, Schwarz MA, Karolczak M, Bruenner TS, Kalender W, et al. In vivo micro-CT imaging of rat brain glioma: a comparison with 3 T MRI and histology. Neurosci Lett. 2009;458(1):28–31.
Giżewski T, Kowalski IM, Zarzycki D, Radomska-Wilczewska A, Lewandowski R, Kotwicki T. Model of self-learning system in medical diagnostics. Pol Ann Med. 2008;15(1):34–42.
Jędrzejewski G. Obrazowanie perfuzji w oparciu o tomografie˛ rezonansu magnetycznego [MRI-based methods for perfusion imaging]. Pol J Radiol. 2006;71(4):52–54.
Jurkowski M, Bobek-Billewicz B. Usefulness of clinical MR scanners for rats brain imaging. Pol J Radiol. 2007;72(1):240.
Królicki L, Zuchowa Z, Andrysiak R. Współczesna diagnostyka obrazowa guzów ośrodkowego układu nerwowego [Contemporary image diagnostic of central nervous system tumors]. Przew Lek. 2003;6(11/12):78–84.
Levene HB, Mohamed FB, Faro SH, Seshadri AB, Loftus CM, Tuma RF, et al. Small mammal MRI imaging in spinal cord injury: a novel practical technique for using a 1.5 T MRI. J Neurosci Methods 2008;172(2):245–249.
Linn J, Schwarz F, Schichor C, Wiesmann M. Cranial MRI of small rodents using a clinical MR scanner. Methods 2007;43(1):2–11.
Nolte I, Pham M, Bendszus M. Experimental nerve imaging at 1.5 T. Methods 2007;43(1):21–28.
Sankowska M, Sa˛siadek M, Sosnowska-Pacuszko D. Współczesna diagnostyka obrazowa guzów wewnątrzkanałowych [Contemporary diagnostic imaging of spinal canal tumors]. Adv Clin Exp Med. 2006;15(4):711–722.
Stoll G, Wesemeier C, Gold R, Solymosi L, Toyka KV, Bendszus M. In vivo monitoring of macrophage infiltration in experimental autoimmune neuritis by magnetic resonance imaging. J Neuroimmunol. 2004;149(1–2):142–146.
Stoll G, Wessig C, Gold R, Bendszus M. Assessment of lesion evolution in experimental autoimmune neuritis by gadofluorine M-enhanced MR neurography. Exp Neurol. 2006;197:150–156.
Thorsen F, Ersland L, Nordli H, Enger PØ, Huszthy PC, Lundervold A, et al. Imaging of experimental rat gliomas using a clinical MR scanner. J Neurooncol. 2003;63(3):225–231.
Ulmer S, Reeh M, Krause J, Herdegen T, Heldt-Feindt J, Jansen O, et al. Dynamic contrast-enhanced susceptibility-weighted per- fusion MRI (DSC-MRI) in a glioma model of the rat brain using a conventional receive-only surface coil with a inner diameter of 47 mm at a clinical 1.5 T scanner. J Neurosci Methods 2008;172(2):168–172.
Yamamoto A, Sato H, Enmi J, Ishida K, Ose T, Kimura A, et al. Use of a clinical MRI scanner for preclinical research on rats. Radiol Phys Technol. 2009;2(1):13–21.
Zwiernik J, Zwiernik B, Cebulski Z, Siwek T. New symptoms in a patient with diagnosed porphyria – untypical clinical course or another disease? Extended differential diagnosis of multiple sclerosis. Pol Ann Med. 2011;18(1):103–109.