Pathophysiological justification of age- and gender-dependent morphological changes in the adipose tissue in rat models of metabolic syndrome
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D. Alpern Phisiological Pathology Department, Kharkiv National Medical University, Kharkiv, Ukraine
Submission date: 2021-01-13
Final revision date: 2021-02-25
Acceptance date: 2021-02-25
Online publication date: 2021-09-24
Corresponding author
Natalia A. Shutova   

Kharkiv National Medical University
Pol. Ann. Med. 2021;28(2):155-161
The mechanisms of metabolic syndrome (MS) is one of the urgent issues in medicine. Regional distribution of the adipose tissue should be diagnosed at clinical examination, as the morphometric parameters of the cells of the active adipose tissue components may indicate the metabolic state.

The aim of the study was to evaluate the differences in morphological and histological parameters of the adipose tissue associated with the development of MS in animals of different ages and gender.

Material and methods:
An experimental study was carried out on 144 WAG/G Sto white rats, divided into three study groups. Group 1 included young immature rats, 3 months old; group 2 consisted of 48 sexually mature rats, aged 5–6 months; group 3 consisted of 48 old rats, 18 months old. Each group was divided into 2 subgroups, control and experimental, and was additionally divided according to gender.

Results and discussion:
The body mass indices and specific weights of mesenteric, epididymal, retroperitoneal and subcutaneous adipose tissue were determined in rats, as well as morphological characteristics of adipocytes of the adipose tissue. It was shown that histological and morphological changes in the adipose tissue of the animals were age- and gender-dependent, and that obesity is associated with chronic inflammation of the adipose tissue.

The results of the study can be used for further determination of possible age and gender differences in the adipose tissue involvement in the development of chronic inflammation, as well as monitoring and correction of adipose tissue dysfunction in MS.

None declared.
None declared.
Smith U, Kahn BB. Adipose tissue regulates insulin sensitivity: role of adipogenesis, de novo lipogenesis and novel lipids. J Intern Med. 2016;280(5):465‒475.
Belenkov YN, Privalova EV, Kaplunova VY, et al. Metabolic syndrome: development of the issue, main diagnostic criteria. Ration Pharmac Card. 2018;14(5):757‒764.
Longo M, Zatterale F, Naderi J, et al. Adipose tissue dysfunction as determinant of obesity-associated metabolic complications. Int J Mol. 2019;20(9):2358.
Mohsen IM. Subcutaneous and visceral adipose tissue: structural and functional differences. Obes Rev. 2010;11(1):11‒18.
Shen W, Wang ZM, Punyanita V, et al. Adipose tissue quantification by imaging methods: A proposed classification. Obes Res. 2003;11(1):5‒16.
Vasiliev VN, Milto IV, Yakimovich IYu, et al. Morphometric parameters of white adipose tissue of different localization in rats on a high-fat diet. Mod Probl Sci Ed. 2015;5:48‒59.
Koterov AN, Ushenkova LN, Zybenkova ES, Winson AA, Biryukov AP, Samoilov AS. The dependence of body weight on age for outbred white and eight lines of laboratory rats: synthetic studies of data from experimental works and nurseries in the aspect of the relationship with radiosensitivity. Some characteristics of the “rat” species. Med Radiol Radiat Saf. 2018;63(2):1‒41.
Rostami H, Tavakoli HR, Yuzbashian E, et al. The association of dietary fat sources with leptin gene expression from visceral and subcutaneous adipose tissues among tehranian adults [in Persian]. Iran J Endoc Metabol. 2018;20(4):160‒168.
Kursawe R, Eszlinger M, Narayan D, et al. Cellularity and adipogenic profile of the abdominal subcutaneous adipose tissue from obese adolescents: association with insulin resistance and hepatic steatosis. Diabetes. 2010;59(9):2288‒2296.
Manolopoulos KN, Karpe F, Frayn KN. Gluteofemoral body fat as a determinant of metabolic health. Int J Obes. 2010;34(6):949‒959.
Zhang M, Hu T, Zhang S, Zhou L. Associations of different adipose tissue depots with insulin resistance: A systematic review and meta-analysis of observational studies. Sci Rep. 2015;5:18495‒18502.
Lopes HF, Corrêa-Giannella ML, Consolim-Colombo FM, Egan BM. Visceral adiposity. Diabet Metabol Syndr. 2016;8:40.
Arner P, Bernard S, Salehpour M, et al. Dynamics of human adipose lipid turnover in health and metabolic disease. Nature. 2011;478(7367):110‒113.
Jo J, Shreif Z, Periwal V. Quantitative dynamics of adipose cells. Adipoc. 2012;1(2):80‒88.
Metwally Ibrahim SEL, Kosba AA. Royal jelly supplementation reduces skeletal muscle lipotoxicity and insulin resistance in aged obese rats. Pathoph. 2018;25(4):307‒315.
Dikar DB, Petersen KF, Schulman GI. Mechanisms of insulin resistance in humans and possible links to inflammation. Hypertens. 2005;45(5):828‒833.
Ferrannini E, Natali A, Bell P, et al. Insulin resistance and hypersecretion in obesity. European Group for the Study of Insulin Resistance (EGIR). JCI. 1997;100(5):1166‒1173.
Dubovyk OS, Mishyna MM. Effect of blue and red spectrum led irradiation on immunocytokine state at purulent-inflammatory processes (experimental research) [in Ukrainian]. Ukr J Med Biol Sport. 2017;4(6):13‒19.
Order of the Ministry of Health of Ukraine No. 690 about the consolidated procedure for conducting clinical VIP tests and examinations of materials of clinical VIP tests and the Standard Provisions on the Committee for Nutrition, on September 23, 2009 [in Ukrainian]. Accessed: 30.11.2020.
Kuzmina IYu, Shutuva NA, Nicolaeva OV. Patent for invention No. 118945 Method of modeling metabolic syndrome in experiments [in Ukrainian]. Ukraine, Zaporizhzhia; 2019: MPK G09B 23/28.
Spalding KL, Arner E, Westermark PO, et al. Dynamics of fat cell turnover in humans. Nature. 2008;453:783‒787.
Verboven K, Wouters K, Gaens K, et al. Abdominal subcutaneous and visceral adipocyte size, lipolysis and inflammation relate to insulin resistance in male obese humans. Sci Rep. 2018;8(1):4677.
McLaughlin T, Craig C, Liu LF, et al. Adipose cell size and regional fat deposition as predictors of metabolic response to overfeeding in insulin resistant and insulin-sensitive humans. Diabet. 2016;65(5):1245‒1254.
Arner E, Westermark PO, Spalding KL, et al. Adipocyte turnover: Relevance to human adipose tissue morphology. Diabet. 2010;59(1):105‒109.
Gomez-Serrano M, Camafeita E, Garcia-Santos E, et al. Proteome-wide alterations on adipose tissue from obese patients as age-, diabetes- and genderspecific hallmarks. Sci Rep. 2016;6:256‒257.
Altman J. Weight in the balance. Neuroendocrinology. 2020;76(3):131‒136.
Sulaeva ON, Belemets NI. Sexual characteristics of adipose tissue regulation [in Ukrainian]. Clin Endocrinol Endocrin Surg. 2017;4(60):11‒20.
Anderson WD, Soh JY, Innis SE, et al. Sex differences in human adipose tissue gene expression and genetic regulation involve adipogenesis. Gen Res. 2020;30(10):1379‒1392.
Gil-Iturbe E, Arbones-Mainar JM, Moreno-Aliaga MJ, Lostao MP. GLUT12 and adipose tissue: Expression, regulation and its relation with obesity in mice. Acta Physiol. 2019;226(4):e13283.
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